FrameStar® 96 Well Skirted PCR Plate

Overview

The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well
This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
This is especially recommended for reaction volumes below 20 µl
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

An Azenta Life Sciences product. For more information please visit azenta.com

Key Features

Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Stackable

Wells

Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
<0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

Frame

Rigid polycarbonate frame for added mechanical stability
Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
Improved seal integrity due to thermal stability of frame
Alphanumeric grid reference to aid well and sample identification

Use

Recommended for low volume PCR
Ideal for use with robotic systems
Compatible with standard multichannel pipettes

Options

Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
Also available as a black frame with black wells for non-PCR fluorescent applications
Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer® Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range
Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
Available barcoded upon request; learn more about our barcoding options
Combi packs available (for 4ti-0960) with Q-Stick™ qPCR Adhesive Seal (4ti-0565)
Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Technical Specifications

Plate length	127.76 ± 0.25 mm
Plate width	85.48 ± 0.25 mm
Plate height	16.10 ± 0.25 mm
Well depth	15.10 ± 0.10 mm
Well diameter	5.50 ± 0.10 mm
Distance to center of A1 from top edge	11.24 ± 0.25 mm
Distance to center of A1 from left edge	14.38 ± 0.25 mm
Pitch (distance between A1 and A2)	9.00 mm

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.

Compatibility

AGILENT/STRATAGENE

Thermal Cyclers (96 well block): Robocycler Gradient

qPCR Cyclers (96 well block): AriaMx

ANALYTIK JENA/BIOMETRA

BIO-RAD

Thermal Cyclers (96 well block): C1000 Touch | S1000

qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect

BIO-RAD MJ RESEARCH

Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™

qPCR Cyclers (96 well block): Chromo4™ | Opticon2™

EPPENDORF

Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1

qPCR Cyclers (96 well block): MasterCycler™ ep realplex

GE HEALTHCARE/AMERSHAM

Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2

PEQLAB/VWR

Thermal Cyclers (96 well block): peqSTAR 96X

SENSOQUEST

Thermal Cyclers (96 well block): Labcycler

TECHNE

qPCR Cyclers (96 well block): Quantica

This product is covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: U.S. Patent Nos. 7,347,977 and 6,340,589.