PURIFIED TEMPLATES

GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences provides reliable, award-winning Sanger DNA Sequencing for purified templates through our Pre-Mixed, Pre-Defined, Custom and Difficult Template sequencing service options. Sanger sequencing from purified templates is our most popular template type. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions.

Ensure Complete Plasmid Validation on Your First Attempt

with Oxford Nanopore Sequencing

SANGER SEQUENCING OF PURIFIED TEMPLATES

Azenta offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom.
To determine the service that is best for your samples, please see the descriptions for each service below.

If you need help choosing your optimal Sanger service level, please contact us:

Email | Phone (1-877-436-3949, Ext. 2) | Live Chat

Pre-Mixed

For sequencing plasmids or purified PCR products, this service is the most economical choice.

You provide your template and primer mixed in the same tube according to our Sample Submission Guidelines.

Pre-Defined

By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a Azenta scientist will mix them when your sample is received. An additional charge applies in most cases.

For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our Sample Submission Guidelines.

Note: An Azenta Universal Primer can be used in this service at no charge.

Custom

This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, an Azenta scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.

Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.

Submit template and primer separately according to our Sample Submission Guidelines.

Note: An Azenta Universal Primer can be used in this service at no charge.

Difficult Template Sequencing

Revolutionize your sequencing experience with our Difficult Template sequencing service, specifically designed for challenging templates featuring GC-rich regions or intricate secondary structures like hairpins. With a success rate surpassing standard workflows by over double, researchers can now save at least a day in their process. Versatile and efficient, this protocol accommodates various DNA types without the need for additional templates, offering unparalleled convenience and reliability.

Learn more about our proprietary Sanger sequencing service – Difficult Template Sequencing

Features & BENEFITS

High Quality

DNA sequencing read lengths ~1000 bases and at least 500 bases Phred20. A typical read will provide 800 bases Phred 20. Quality Score and Contiguous Read Length (CRL) provided in each read

Convenient

Best-in-Class Difficult Template Protocols

For hairpins and GC-rich DNA

Free Vector Primers

Wide selection of Azenta Universal Primers are available for most standard vectors

Technical Support

Available at no charge to discuss your project, troubleshoot, and answer questions 8 AM – 8 PM Eastern Time, Monday – Friday; results are posted on Saturdays for weekend workers

Online System

24/7 web-based online ordering and data retrieval, with bar-coded order tracking

Technology

Analysis using ABI 3730xl DNA Analyzers for capillary electrophoresis and fluorescent dye terminator detection

TECHNICAL RESOURCES

Sanger Sequencing Quick Tips Guide

Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template

Blog | Troubleshooting DNA Templates with Sanger Sequencing

Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.

Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing

Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.

Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.

Blog | Analyzing Sanger Sequencing Data

The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.