We accept PCR products (crude or purified), RNA, cDNA, cell pellets, fresh/frozen tissue samples, and viral particles.

Yes, for confirmatory sequencing a reference sequence is required. If you do not want to share your reference sequence with us, GLP-grade Standalone Sanger Sequencing may fit your needs.

Yes, we can design the assay(s) to target the region of interest based on the provided reference sequence.

You will receive a GLP-grade study report reviewed by the Azenta Quality Assurance department which includes strategies used, consensus sequences, and any sequence discrepancies detected by comparison to the reference sequences. We will also provide raw sequencing data in .ab1, .phd, and .seq formats, a signed GLP Compliance Statement provided by the Study Director, and a signed Quality Assurance Statement listing project inspection and reporting dates.

SNP/Mutation Analysis is a PCR plus sanger sequencing technique targeted toward analyzing Single Nucleotide Polymorphisms (SNPs) and mutations at the genomic DNA level.

We accept Purified gDNA, cell pellets, fresh/frozen tissue samples, yeast colonies, and bacterial colonies.

Yes, we can design assays to target your region of interest. To do so, we request either the accession number, the chromosome position along with genome build, the name of the gene of interest, or SNP ID (rs#).

You will receive a GLP-grade study report reviewed by the Azenta Quality Assurance department which includes strategies used, consensus sequences, and any sequence discrepancies detected by comparison to the reference sequences. We will also provide raw sequencing data in .ab1, .phd, and .seq formats, a signed GLP Compliance Statement provided by the Study Director, and a signed Quality Assurance Statement listing project inspection and reporting dates.

We accept Purified PCR products (Pre-Mixed or Pre-Defined reactions) and Purified Plasmid DNA (Pre-Mixed or Pre-Defined reactions).

A “Pre-mixed” reaction is submitted with DNA at an adjusted sample concentration that either already contains sequencing primers from the customer, or will use a free Azenta universal primer. Azenta sample submission guidelines must be followed with this method .

A “Pre-defined” reaction is submitted with DNA at an adjusted sample concentration that does not contain any sequencing primers. You will need to supply your primer in a separate tube for Azenta to add to your reaction. Azenta sample submission guidelines must be followed with this method.

No. For standalone sequencing, only material that can be directly sequenced can be submitted. If PCR is needed prior to sequencing, our Confirmatory Sanger Sequencing service line may fit your needs.

You will receive a GLP-grade study report reviewed by the Azenta Quality Assurance department which includes quality scores of all sequencing traces, raw sequencing data in .ab1, .phd, and .seq format, a signed GLP Compliance Statement provided by the Study Director, and a signed Quality Assurance Statement listing project inspection and reporting dates.

To confirm plasmid identity after preparation, the purified plasmid will be analyzed through restriction digest and sanger sequencing.

A GLP-grade study report reviewed by the Azenta Quality Assurance department which includes strategies used, consensus sequences, and any sequence discrepancies detected by comparison to the reference sequences. You will also receive raw sequencing data in .ab1, .phd, and .seq formats, a signed GLP Compliance Statement provided by the Study Director, and a signed Quality Assurance Statement listing project inspection and reporting dates. A custom certificate of analysis (CoA) will also be included with the plasmid preparation.

We accept cell pellets, fresh/frozen tissue samples, and viral particles.

You will receive a GLP-grade study report reviewed by the Azenta Quality Assurance department, a signed GLP Compliance Statement provided by the Study Director, and a signed Quality Assurance Statement listing project inspection and reporting dates.