Overnight DNA Oligos: Standard (non-modified, desalted) DNA oligos nucleotides (ranging from 15-60 bases at 25 or 50 nmol) delivered in 1-2 business days with two priorities options:
Please click here to learn more about our Overnight DNA Oligos service and for guidelines based on region, oligo length, and time of order.
Custom DNA & RNA Oligos: Synthesis of custom-length oligos (5-150 bases) at 25 - 1000 nmol scales with a wide range of modifications available with HPLC, PAGE, and desalted purification options. Turnaround time starts at 4 business days, depending on scale and modification requirements.
Oligos Retained for Sanger: Order oligos for your Azenta Sanger sequencing order. Azenta will synthesize, dilute, and store the oligo on-site for up to 1 year.
IUPAC Code | Base |
---|---|
R | A or G |
Y | C or T |
M | A or C |
K | G or T |
S | C or G |
W | A or T |
H | A, C, or T |
B | C, G, or T |
V | A, C, or G |
D | A, G, or T |
N | A, C, G, or T |
Overnight DNA Oligos orders are typically received within 1-2 business days. Please reference delivery times here.
Custom DNA & RNA Oligos service ships orders in as few as 4 business days depending on the modifications or purifications requested. Please refer to your order for specific turnaround time information.
Oligos Retained for Sanger will be available for sequencing within 1-2 business days depending on the time the order is placed.
Overnight DNA Oligo orders can be delivered dry or formulated dry, wet, in water, or a selection of buffers, normalized to 50 or 100 uM for no additional charge.
Custom DNA & RNA Oligo orders can only be delivered in dry format.
Purification | Lengths (nt) | Purity | Recommended Uses |
---|---|---|---|
Desalted | 5-90 | >70% | Standard sequencing and PCR primers |
PAGE | 11-150 | >90% | Long oligos |
HPLC | 5-90 | >95% | Modified oligos or oligos used in sensitive applications |
Length (nt) | Scale | Purification | Target Yield* |
---|---|---|---|
5-90 | 25 nmol | Desalted | 5 nmol |
HPLC | 2 nmol | ||
50 nmol | Desalted | 10 nmol | |
HPLC | 4 nmol | ||
PAGE | 2 nmol | ||
100 nmol | Desalted | 20 nmol | |
HPLC | 8 nmol | ||
PAGE | 4 nmol | ||
200 nmol | Desalted | 50 nmol | |
HPLC | 20 nmol | ||
PAGE | 10 nmol | ||
1 µmol | Desalted | 300 nmol | |
HPLC | 120 nmol | ||
PAGE | 60 nmol | ||
91-150 | 100 nmol | PAGE | 2 nmol |
200 nmol | PAGE | 5 nmol | |
1 µmol | PAGE | 25 nmol |
Dye | Max Ex (nm) | Max Em (nm) | Color | Compatible Quenchers |
---|---|---|---|---|
6-FAM | 495 | 520 | Green | BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl |
JOE | 520 | 548 | Orange | BHQ-1, ECLIPSE, Dabcyl, TAMRA |
TET™ | 521 | 536 | Orange | BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl |
HEX™ | 520 | 548 | Pink | BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl |
TAMRA | 544 | 576 | Rose | BHQ-2, ECLIPSE, Dabcyl |
ROX™ | 576 | 601 | Red | BHQ-2, ECLIPSE |
Cy®5 | 650 | 670 | Violet | BHQ-2 |
Cy®3 | 550 | 570 | Red | BHQ-2, ECLIPSE |
TexasRed® | 583 | 603 | Red | BHQ-2, ECLIPSE |