Tools for Viewing Sanger Sequencing Data

Sequence / Chromatogram Viewing Software

A number of free software programs are available for viewing trace or chromatogram files. Click on the appropriate icon(s) to go to the respective Web page. You will find information about downloading, installing and using the software. Please make your choice based on your computer platform and operating system.

Sanger Data Analysis


Free/Paid Trace File Viewer Multiple Sequence Alignment & Contig Assembly Windows Mac Linux In-browser
4Peaks Free
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Sequence Scanner Free
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Chromas Lite Free
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SnapGene Viewer Free
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GeneStudio Pro Free


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UGENE Free




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Chromaseq Free




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BioLign
(Note: See bottom half of page for description and download link)
Free -

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CAP3
(Note: Only for contig assembly of sequences in FASTA format)
Free -
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Geneious Paid




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SeqMan Pro Paid



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Sequencher Paid



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Vector NTI Express Paid



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Chromas Pro Paid


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CodonCode Aligner Paid



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DNA Baser Paid


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Free Molecular Cloning Software


Windows Mac Linux
SnapGene Viewer
Serial Cloner
UGENE
ApE (A Plasmid Editor) -

Decompression Software

WinZip

Free Online Primer Design Software

Primer3
NCBI Primer-BLAST

Free Online Oligo Analysis Tools

Oligo Calc