ENZYMATIC CONVERSION & SEQUENCING
1. Protection/Enzymatic Conversion
Submit cells, tissue, or gDNA for enzymatic conversion of unprotected cytosine
2. Library Preparation
Add adapters to ssDNA fragments
3. Sequencing
Sequence 2x150 bp on Illumina® platform
4. Data Analysis
Identify 5mC sites, calculate methylation ratios, and perform differential methylation analysis