Immune Profiling

Immune profiling, also known as immunoprofiling, couples ultra-deep sequencing of V(D)J regions and customized bioinformatics to provide comprehensive characterization of the entire B-cell receptor (BCR) or T-cell receptor (TCR) repertoire. In addition, recent advances in microfluidic technology allow BCR and TCR chains to be paired at single-cell resolution. The immense diversity of BCRs and TCRs, generated through recombination of the variable (V), diversity (D), and joining (J) loci, is key to the adaptive immune system.
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What is immune profiling?

Immune profiling is the systemic identification and quantification of immune cells and their functional attributes within a tissue or organism. In immune profiling, T cells and B cells bind specific antigens, proliferate, and trigger a specific immune response. The T-cell and B-cell receptors are then analyzed to examine mechanisms of autoimmune disease, cellular development, and immune-oncology therapies.

What is single-cell immune profiling?

Single-cell immune profiling is the collective analysis of BCRs and TCRs, antigen specificity, protein expression, and gene expression of an individual cell.

Immune Profiling Service Options

Analyze V(D)J sequences at the RNA level using bulk or single-cell approaches.

	Immuno-Profiling for bulk repertoire analysis	Single-Cell Immuno-Profiling for physical pairing of chains
BCR sequencing (VH and VL chains)	Yes	Yes
TCR Sequencing (α and β chains)	Yes	Yes
Starting materials*	Total RNA Blood Cell pellet Tissue	Cell suspension
V(D)J amplification method	Proprietary 5' RACE	Targeted enrichment of cDNA with cell-specified barcodes
Physical pairing of VH/VL or α/β chains	No	Yes

*Visit our Sample Submissions Guidelines for more details.

Workflow

Features & Benefits

BCR and TCR analysis available for multiple species, including human and mouse

Easy-to-interpret reports enable in-depth data analysis

Flexible sample requirements facilitate project design

Ph.D.-level consultation provides guidance before, during and after the project

Industry-leading turnaround time

Comprehensive research solutions with downstream synthetic DNA libraries available

Technical Resources

Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System

In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with GENEWIZ developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences.