GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences provides reliable, award-winning Sanger DNA Sequencing for purified templates through our Pre-Mixed, Pre-Defined, Custom and Difficult Template sequencing service options. Sanger sequencing from purified templates is our most popular template type. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions.
Azenta offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom.
To determine the service that is best for your samples, please see the descriptions for each service below.
If you need help choosing your optimal Sanger service level, please contact us:
For sequencing plasmids or purified PCR products, this service is the most economical choice.
You provide your template and primer mixed in the same tube according to our Sample Submission Guidelines.
By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a Azenta scientist will mix them when your sample is received. An additional charge applies in most cases.
For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our Sample Submission Guidelines.
Note: An Azenta Universal Primer can be used in this service at no charge.
This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, an Azenta scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.
Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.
Submit template and primer separately according to our Sample Submission Guidelines.
Note: An Azenta Universal Primer can be used in this service at no charge.
Revolutionize your sequencing experience with our Difficult Template sequencing service, specifically designed for challenging templates featuring GC-rich regions or intricate secondary structures like hairpins. With a success rate surpassing standard workflows by over double, researchers can now save at least a day in their process. Versatile and efficient, this protocol accommodates various DNA types without the need for additional templates, offering unparalleled convenience and reliability.
Learn more about our proprietary Sanger sequencing service – Difficult Template Sequencing
Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template
Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.
Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data.
*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.