Custom Short-Read Amplicon Sequencing

Azenta Life Sciences, formerly GENEWIZ routinely sequences pre-made amplicons on Illumina platforms to answer a myriad of biological questions. Standard amplicon sequencing provides flexible data requirements for amplicons starting at 100 bp in size. Amplicons can either be fragmented or sequenced contiguously depending on their size and the purpose of your project.

Applications for this service span many different research areas. Scientists commonly sequence amplicons for antibody library screening, natural antibody repertoire profiling, disease genotyping, genome editing (i.e. CRISPR) mutation analysis, screening of molecular clones, and analysis of plasmid stocks – to name a few.

Features & Benefits

Flexible data amount options

Fastest turnaround time in the market with 99% on-time delivery

Extensive multiplexing flexibility (up to 10K samples/run)

Optimized processes- exceed manufacturer’s specifications for data amount and quality

Ability to process your amplicons to remove excess primers and/or off-target products

Custom platform configurations and sequencing/indexing primers available

Sample Submission Guidelines

Azenta accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.

Deliverables

All customers receive their raw data as FASTQ files. Customizable data analysis packages are available by request.

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.