Metagenomic sequencing uses next generation sequencing (NGS) to rapidly analyze microbial community diversity and understand the role of constituent species. Our metagenomic sequencing solutions range from targeted assays, such as 16S for prokaryotes and ITS2 for fungi, to shotgun sequencing and metagenomic analysis. Explore below which service best fits your needs, or check out our webinar, “Uncovering Hidden Microbial Diversity with NGS Technology,” to learn more about our capabilities.
Metagenomic sequencing is the collective analysis of all genomic material present in a sample (containing mixed microorganism communities), thus eliminating the need for isolating and cultivating individual organisms.
Both metagenomics and genomics are fields of study within molecular biology, but the difference is in scope. While genomics is focused on the genome of a single organism, metagenomics encompasses all genomes of various microorganisms within a single given sample, providing a broad overview of an ecosystem’s composition.
16S-EZ | Fungal Identification | Whole Genome Metagenomics | |
Microbes Targeted |
Bacteria and Archaea | Fungi | All |
Taxon Level |
Genus/Species | Genus/Species | Sub-species |
Genomic Loci Sequenced | 16S rRNA gene (V3 and V4 regions) |
Internal Transcribed Spacer 2 (ITS2) |
Whole Genomes |
Purpose of Assay | Taxon Identification |
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Benefits | Enhanced sensitivity outperforms V4-only assays |
High variation in ITS2 region allows differentiation between closely related species |
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Available Reports |
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This webinar outlines the role and importance of microorganisms in our everyday world, how to classify and identify them, and the next generation sequencing techniques that work best for these tasks. We touch on the wide array of areas that microbial genomics can be applied to and the NGS case studies that have helped scientists thrive in various research areas.
Watch NowWe compared GENEWIZ’s 16S MetaVx Environmental service against a traditional V4-only assay. 16S MetaVx Environmental consistently detected a greater number of bacteria and archaea, demonstrating its superior sensitivity and specificity.
Download NowThe rapid and wide-spread evolution of antibiotic resistance is threatening global health. In this webinar, presented by Dr. Mato Lagator, Wellcome Trust, Royal Society Sir Henry Dale Fellow, University of Manchester, you will learn about different approaches to study evolutionary processes that underpin the emergence of resistance. Special focus is given on how these methods can be utilized to improve drug development and longevity.
Watch NowGENEWIZ accepts a variety of starting materials for Metagenomics projects. For detailed sample submission requirements please visit our Sample Submission Guidelines page.
All customers receive their raw data as FASTQ files. MetaVx2.0™ provides an enhanced report through analysis with the QIIME software suite. This optional, enhanced report includes figures such as rank-abundance curves, taxonomic classification, rarefaction curves, principal coordinate analysis and hierarchical clustering analysis. An interactive taxonomic viewer detailing different taxa and their relative abundance in each sample is also available by request.
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.