Whole Genome Sequencing

Whole genome sequencing (WGS) provides unprecedented access to genomic information, accelerating breakthroughs in human healthcare, oncology, biomarker discovery, agriculture, and metagenomics.

Our whole genome sequencing services use cutting-edge technologies and bioinformatics to deliver high-quality data and analysis for your genome of interest. Whether you are studying human, animal, plant, bacteria, or virus species, we have a range of WGS services to cater to your unique research needs, no matter your desired read length or coverage.

Whole Genome Sequencing Services

Short-Read Human WGS

Analyze human genomes with speed and accuracy using the Illumina® NovaSeq™ or HiSeq®. CLIA-compliant whole genome sequencing is available for clinical researchers.

Short-Read Non-Human WGS

Perform de novo assembly and variant detection on the Illumina® MiSeq®, HiSeq®, or NovaSeq™ with optimal throughput and low cost per base. Extraction through data analysis is available.

Long-Read Whole Genome Sequencing

Long-read sequencing on the PacBio® Sequel® delivers up to 60 kb reads with contig N50 > 1 Mb. Generate reference quality assemblies for prokaryotic genomes. Ideal for structural variant calling in eukaryotes.

Low-Pass Whole Genome Sequencing

Accurately detect genetic variation throughout genomes of a multitude of species. Achieve up to 99% accuracy of variant call detection at a very low sequence coverage (0.1X<10X) using imputation algorithms.

What is whole genome sequencing and what is it used for?

Whole genome sequencing (WGS) is the comprehensive read and analysis of an entire genome, including non-coding regions of the genome.

WGS is used to facilitate discovery of novel genes and gene variants associated with disease. In the case of human WGS, researchers use WGS to explore gene expression and functional elements of the genome that help predict an individual’s response to drug therapies. WGS is also used to study the evolution of infectious pathogens and mechanisms of disease-causing mutation. Find the right NGS solution for your project using our interactive guide.

WGS coverage for any application

Features & Benefits

Superior Data Quality

Exceeding manufacturer’s benchmarks
Fast Turnaround

Starting at 1 week for sequencing
US-Based Processing

Sequencing and customer support in New Jersey

Dedicated Ph.D.-level support at every step with real-time project updates through our online system

Complete sequencing solutions from extraction to data analysis with many sample types accepted

Automated workflows increase scalability and improve reproducibility

Single-cell whole genome sequencing with CNV profiling available on the 10x Genomics platform

More reasons to choose Azenta

NEW Direct Colony to
Whole Plasmid Sequencing

Technical Resources

Quick Start Guide | Exploring Bioinformatics for Genome and Transcriptome Sequencing Data

For those new to bioinformatics, analyzing massive amounts of NGS data can be a daunting task. Download Azenta’s bioinformatics quick start guide to learn how to analyze whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) data with bioinformatics tools to reveal biological insights for your research.

Case Study | Detection of Previously Unidentified Structural Variants Involved in Glioblastoma Using PacBio^® Sequel^® Sequencing

This case study looks at how Azenta utilized the PacBio Sequel long-read technology to help a customer improve detection of structural variants within glioblastoma samples.

Webinar | A New Paradigm in DNA Sequencing – Highly Accurate Single-Molecule Long Reads (Hosted by PacBio)

In this PacBio-hosted webinar, Jonas Korlach, PacBio Chief Scientific Officer, and Dave Corney, Azenta Associate Principal Scientist, Next Generation Sequencing, describe the recent release of Sequel System 6.0, which has revolutionized long-read sequencing by providing users the ability to generate highly accurate single-molecule reads.

Tech Note | Multiplexing Microbes on the PacBio Sequel: Feasibility, Benefits, and Recent Developments

This study proves the feasibility of multiplexing and highlights the key advantages of the PacBio Sequel platform as compared to its predecessor, the RSII.

Best Practices | Preparation of High Molecular Weight DNA

High-quality high molecular weight (HMW) genomic DNA (≥50 kb) is critical to achieving long read lengths on platforms such as the 10x Genomics^® Chromium™ and the PacBio Sequel. Follow these guidelines to generate the best possible WGS results.

The $100 Genome: Using Low-Pass Whole Genome Sequencing as an Alternative to Genotyping Arrays

In this webinar originally presented at the ASHG 2020 Virtual Meeting, learn how low-pass WGS overcomes the inherent limitations and biases of traditional arrays, offering an inexpensive, high-throughput alternative for detecting genome-wide genetic variation and novel variants.

Workshop & Roundtable Discussion | Exploring Bioinformatics for Whole Genome Sequencing (WGS) Data

In this recording of our WGS bioinformatics workshop & roundtable discussion led by bioinformatics scientist Zain Alvi, Ph.D., we'll guide you through the WGS bioinformatics process to help you learn to interpret WGS bioinformatics results, as well as address common challenges and answer frequently asked questions (FAQs).

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.

Whole Genome Sequencing