Targeted Sequencing

Targeted sequencing assay use next generation sequencing (NGS) to analyze your specific gene(s) of interest. Whether you want to pinpoint a few genes or a few hundred, our custom targeted sequencing panels offer high sensitivity and specificity, providing in-depth coverage and high-quality data to discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.

To investigate the location and frequency of vector integration in the host genome following transduction, use our integration site analysis (ISA) solution for pre-clinical and clinical cell and gene therapy research.

What is targeted sequencing?

Targeted sequencing is a technique that uses NGS to target specific genes, coding regions of the genome, or chromosomal segments for rapid identification and analysis of genetic mutations.

Why use targeted sequencing?

Targeted sequencing is useful for studying gene variants in selected genes or specific regions of the genome, since a large diversity of organisms is targeted rapidly and cost-effectively. Targeted sequencing is used to examine gene interactions in specific pathways and is faster and more cost effective than whole genome sequencing (WGS). This is because a smaller set of nucleotides are used, rather than broadly sequencing the entire genome.

Targeted sequencing Gene Panels

GENEWIZ from Azenta provides a variety of targeted resequencing panels, either pre-made or custom, that can cover any genes of interest or cancer-implicated genes.

Visit these pages to learn more about these services:

Targeted Sequencing Applications

Discover genetic biomarkers associated with disease state

Characterize genetic associations to a particular phenotype, such as drug response

Genotype and classify eukaryotic and/or prokaryotic cell lines

Design genetic tests, such as for pharmacogenomics or oncology

Targeted Sequencing Features & Benefits

Extensive multiplexing flexibility enables a more cost-effective assay

Dedicated, on-going support from experienced Azenta scientists

In-house pipeline for optional Sanger-based confirmatory sequencing of final targets utilizing your NGS samples

Comprehensive bioinformatics options for robust downstream analysis

Availability of the most cutting-edge sequencing platforms

Workflow for Targeted Resequencing Panels

Extensive Quality Control is Performed at the Conclusion of Each Step of the Process

Experimental Design

1
Targeting and Library Preparation

2
Sequencing

3
Bioinformatics

4

Sample Submission Guidelines

Azenta accepts the following sample types for targeted resequencing projects:

Purified genomic DNA
Frozen cell pellets (mammalian or bacterial)
PCR amplicons
Bacterial colonies

Formalin-fixed, paraffin-embedded (FFPE) thin sections (slides)
Select tissues
Blood
Swab

NEW Direct Colony to
Whole Plasmid Sequencing

Technical Resources

Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.

Webinar| Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response

Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response.

Webinar | Demystifying End-to-End DNA Sequencing Solutions

In this co-hosted webinar with Twist Bioscience, learn how researchers design custom panels to sequence deeply and efficiently into regions, genes, and organisms of interest, as well as gain a greater understanding on how targeted sequencing can be combined with the latest transcriptomics and proteomics tools to reveal even more insights.

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.