PLASMID DNA PREPARATION

Delivering high quality DNA from a variety of starting materials in a cost-effective and reliable manner is our priority. Don't waste your valuable time with a plasmid preparation kit when GENEWIZ^TM Plasmid Preparation Services from Azenta can conveniently provide plasmid DNA preps at the scale and quantity you need.

Our standard plasmid DNA preparation service is a flexible and fast choice for a range of plasmid templates. Runs are available in the classic mini to giga scales, yielding micrograms to milligrams of purified plasmid DNA. Additional add-on service and analysis options are available, enabling project customization to meet budgetary and technical specifications.

Features & Benefits

No plasmid purification kit needed – Submit starting material and Azenta will deliver your purified plasmid product

Fast delivery time - Rapid turnaround time, starting at just two business days, to meet your research deadlines

Convenient online ordering - Place an order directly through your Azenta account, including your Sanger sequencing results page

Quality control – Strict QC process with multiple analysis options to ensure high-quality plasmid prep

Plasmid DNA Prep Service & Analysis Options

= Included

= Add-on

Turnaround Time	Accepted Plasmids	Service Options	Analysis Options
Starting from 2-3 business days	High- or Low-Copy Toxic Construct Stable or Unstable	Spectrophotometric DNA Quality Analysis Minimum Yield Verification Transfection-Grade (Endotoxin Free) DNA Normalization Aliquots or Custom Tubes GLP-Compliant	DNA Sequencing Sequence Consensus Assembly & Analysis Full-Service DNA Sequence Confirmation Restriction Digest & Agarose Gel Analysis Sterility Testing

Not all options available for mini-scale standard and 96-well preps. Please contact GS@azenta.com to discuss your miniprep project.

Upgrade to our amount based Targeted Yield Service. Transfection-grade and value-added analysis options included.

DNA PREPARATION SCALES AND YIELDS

Run Scale	Typical Yield for High-Copy, Stable Plasmids^	Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^
Midi	70-100 μg	10-50 μg
Maxi	350-500 μg	50-250 μg
Mega	1.5-2.5 mg	250 μg-1.25 mg
Giga	7-10 mg	1-5 mg

Mini Preps	Typical Yield for High-Copy, Stable Plasmids^	Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^
Tube	7-10 μg	2-5 μg
96-well Plates	5-10 μg	1-3 μg

^Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors. Please contact GS@azenta.com to discuss known low-copy, unstable, or toxic constructs.

Choice of Starting Material

DNA

Transformation provided as a service*

Glycerol Stock

Provide 200 μL frozen stock

Bacterial Colony

Please provide fresh colonies (<3 days old)

*For DNA samples, Azenta Life Sciences, formerly GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.

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Related Services

Technical Resources

Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that
direct-sequencing templates are not available for our Same Day service.