ATAC-Seq

ATAC-Seq (assay for transposase-accessible chromatin sequencing) identifies open chromatin regions and efficiently labels them for high-throughput sequencing. This technique has a higher signal-to-noise ratio and lower input requirements than similar approaches.

Our ATAC-Seq service, from library preparation to data analysis, provides an optimized approach for nuclei extraction from cryopreserved cells to ensure detailed insight into genome-wide chromatin accessibility. If you want to understand gene expression regulation at the individual-cell level in varied states and cellular processes, we also offer single-cell ATAC-Seq (scATAC-Seq).

ATAC-Seq Workflow

1. Sample Preparation

Isolate and freeze cells with protocol provided by Azenta

2. Dead Cell Removal

If low viability, live cells are enriched using a magnetic bead-based approach

3. Nucleic Extraction & Library Preparation

Optimized ATAC assay produces robust signal from low inputs

4. Sequencing

Guaranteed data quality exceeds Illumina® benchmarks

5. Bioinformatics

Alignment, peak calling, and multiomic data integration

ATAC-Seq Features & Benefits

Convenient sample preparation – freeze and store cells prior to submission; fresh cells or isolated nuclei are not required

Improved data quality – higher signal-to-noise ratio compared to traditional ATAC-Seq

Flexible starting material – submit as few as 50,000 cells; dead cell removal improves samples with low viability

Single-cell analysis available using the 10x Genomics® Chromium™ platform

More reasons to choose Azenta

ATAC-Seq Overview

Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs a hyperactive form of Tn5 transposase to identify regions of open chromatin, which are important for global epigenetic control of gene expression. Tn5 simultaneously cleaves and adds adapters to nucleosome-free regions of DNA, priming them for sequencing.

Technical Resources

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.