Immune Profiling for Immunogenomics

Immune profiling using next generation sequencing (NGS) allows for ultra-deep immune repertoire analysis. This approach uncovers the composition of B-cell receptors (BCRs) and T-cell receptors (TCRs) generated through the recombination of the V(D)J loci, which is crucial to the adaptive immune system.

For in vivo natural antibody populations, we offer single-cell sequencing for physical pairing of the VH/VL or α/β chains of each complex or bulk sequencing for non-physical pairing inferred through frequency analysis. For in vitro recombinant antibody populations, short- and long-read options are available for unpaired and paired analyses. Add custom bioinformatics to any configuration to comprehensively characterize the entire BCR or TCR repertoire.

Immunosequencing Options

Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.

Antibody Discovery Platform	Method of Pairing Chain Sequences		Sequence Target	Applications
Antibody Discovery Platform	Frequency Analysis	Physical Pairing	Sequence Target	Applications
In vivo	Immuno-Profiling	Single-Cell Immuno-Profiling	B-cell receptors (BCR) T-cell receptors (TCR)	BCR/TCR repertoire profiling In vivo antibody discovery Hybridoma screening
In vitro	Short-Read Amplicon Sequencing	Long-Read Amplicon Sequencing	Antigen-binding fragments (Fab) Single-chain variable fragments (scFv)	In vitro antibody discovery via surface display

Immunosequencing Workflows

Immuno-Profiling

Perform bulk analysis of V(D)J sequences at the RNA level.

Short-Read Amplicon Sequencing

Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:

Single-Cell Immuno-Profiling

Pair BCR and TCR chains at single-cell resolution using 10x Genomics™ technology.

Long-Read Amplicon Sequencing

Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.

TECHNICAL RESOURCES

An Optimized Approach to PBMC Isolation and Cryopreservation

The quantity and quality of isolated cells are critical to the performance of functional assays. In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation leading to reproducible, high-quality results.

Tech Note: Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening

High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.