Plasmid Preparation

Viral Plasmid Containing the Inverted Terminal Repeat (ITR)

Be aware that sequencing the ITR is challenging, so we request concentrated stocks of DNA.

• Optimal sequencing concentration for viral plasmid is 300 ng/µl (minimum 200 ng/µl). Please submit as much template as you are able, up to 10 µl but no less than 5 µl.
• Please include exact concentration in the Notes field for each template.
• Ensure 260/230 ratio of 1.8-2.2, as this protocol is particularly sensitive to organic contamination.
• Primer – Please provide 30 µl per reaction at 5 pmol/µl (5 µM) in a labeled 1.5 or 0.5 ml tube.
• Generate primers that bind between 150 bps and 350 bp from the ITR.

Contact

DNA prep services in Europe**

GENEWIZ Germany GmbH
Bahnhofstr. 86
04158 Leipzig, D-Germany
Tel (EU): +49 (0)341 520 122 – 41
Tel (France): 0811 230001 (Toll-Free)

GENEWIZ Dropbox:
Starting material that is stable at room temperature may be submitted via a GENEWIZ Dropbox where available. A printout of the online Order Summary page must be included with the material. Contact logistics.europe@azenta.com to request a dropbox pick up for your location or if you have any dropbox questions.

Direct Shipping:
If a dropbox is not available or if your starting material requires dry ice, please use a local, overnight delivery service (such as DHL). A printout of the online Order Summary page must be included with the material.

**100 mg to 5 g DNA prep services are processed in operation sites outside of Europe.