ANTIBODY DISCOVERY

Our Precision Antibody Discovery service rapidly identifies and expresses antibody sequences with a broad affinity range for your target and the most promising biophysical profiles for commercialization. Utilizing next generation sequencing (NGS) of your in vivo samples (i.e., B cells, PBMCs) or in vitro libraries (i.e., phage display) and the power of machine learning with our novel bioinformatic platform, AbXtract™, powered by OpenEye and developed by Specifica, this end-to-end workflow enables high-throughput screening of your library to generate 5 – 50x more antibody leads than traditional low-throughput approaches. This results in more functionally diverse leads, even including clonotypes and rare clones, improving antibody program success.

ANTIBODY DISCOVERY SERVICE

Selecting the right antibody leads with favorable development characteristics the first time around is essential for the success of your antibody program. Combining NGS, machine learning bioinformatics, and recombinant antibody production into a singular workflow, GENEWIZ Precision Antibody Discovery empowers you to rapidly and efficiently discover candidates that you would otherwise miss with traditional screening technologies and processes.

Uncover more leads: Increase the number of clonotype leads by 5–10x compared to random colony screening and produce a set of recombinant antibodies ready for experimentation

Achieve greater library diversity: Leveraging NGS, explore the complete antibody repertoire, including rare clones, which are typically missed using low-throughput methods

Decrease sequence liabilities: NGS & computational processing help to reduce sequence liabilities and improve efficiency of downstream therapeutic development, resulting in prioritized leads based on favored developability and biophysical profiles

Discover antibodies faster: Computational processing of NGS datasets coupled with recombinant production provides a faster and more efficient screening process and production of high affinity antibody leads

ANTIBODY DISCOVERY WORKFLOW

BENEFITS OF ANTIBODY DISCOVERY USING ABXTRACT

1. Annotation

Annotate CDRs and framework regions of interest (ROI)
2. Abundance / Enrichment

Enrichment based on your ROI
3. Clustering

Maximize sequence diversity by clustering based on ROI
4. Prioritization

Quantify sequence-based biophysical liabilities and prioritize favorable leads providing immediate gene synthesis-ready output

DELIVERABLES

Standard Deliverables

• NGS FASTQ files
• AbXtract report
• Expressed antibodies (mL to L expression scales available)
• Antibody QC report including SDS-PAGE analysis

TECHNICAL RESOURCES

Application Note | Accelerating Recombinant Antibody Production

Recombinant antibody production is a cornerstone of modern biotechnology and research, but it faces the significant challenge of achieving high yields. In this application note, discover how to enhance expression efficiency while reducing production times with an optimized vector backbone workflow.

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.