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Common methods used for identifying bacterial and fungal strains are 16S rRNA gene sequencing and Internal Transcribed Spacer (ITS) sequencing respectively. These highly conserved regions are standard tools used to construct bacterial and fungal phylogenies and taxonomies. GENEWIZ offers pre-developed PCR assays that cover essential regions within the 16S rRNA gene and the ribosomal ITS region used for amplification and Sanger sequencing of clonal samples.
Service Overview
New! Alignment and analysis of 16S raw data now available. For more information contact GENEWIZ Customer Support
16S/ITS rRNA SERVICE DETAILS
- PCR amplification directly from bacterial or fungal colonies
- Enzymatic purification of crude PCR product enhances sequencing quality
- Forward and reverse sequencing of the 16S rRNA gene and the ITS region
- Starting materials accepted:
- Bacterial and fungal colonies
- Extracted gDNA
- Glycerol stocks
- Cell pellets
FEATURES & BENEFITS
High Quality: Robust 16S rRNA and ITS PCR protocols and high quality sequencing data
Fast & Reliable Turnaround: Data delivery starting at just 2 business days
Industry-leading Customer Service: Easily accessible, dedicated customer service available Monday – Friday
Convenience: Pre-developed assays enable researchers to submit clonal samples for PCR and Sanger sequencing
16S rRNA/ITS Sequencing
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Submit clonal sample
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PCR
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Sanger
Sequencing
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Colony is picked from plate for PCR amplification.
Gene coding for 16S rRNA is amplified by PCR using gene-specific primers. Quality control: PCR product is observed by agarose gel electrophoresis.
Resulting PCR product is sequenced in both directions.
GENEWIZ from Azenta Life Sciences
European GENEWIZ Headquarters
GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41
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