Epigenetic sequencing, or epigenome sequencing, uses next generation sequencing (NGS) assays to analyze the epigenome rapidly and thoroughly. The epigenome encompasses chemical modifications to DNA and histone proteins, chromatin accessibility, and higher-order DNA organization. Its profound effect on gene regulation, and consequently development and pathogenesis, is still being explored. Although epigenetic modifications do not alter the nucleic acid sequence itself, sequencing techniques can detect them indirectly.
We offer epigenetic sequencing solutions such as methylation sequencing, ATAC-Seq, and ChIP-Seq for various sample types, including plant and animal samples.
Epigenomics is the study of reversible modifications in a cell’s DNA that regulate gene expression and mark the genome (turning the gene “on” or “off”) without altering the underlying DNA sequence itself. The field of epigenomics provides insight into complex cellular diseases and how cellular phenotypes are regulated by gene expression dynamics.
Epigenetic sequencing is the use of high-throughput sequencing technology to quantify and analyze DNA modifications that are involved in gene expression and regulation of cell differentiation and development.
To perform epigenetic sequencing, DNA methylation must be preserved and transformed into quantitative signals for genome-wide mapping. There are several methods of epigenetic sequencing; bisulfite sequencing (BS-Seq) and immunoprecipitation (methylated DNA immunoprecipitation or MeDIP) are commonly used in conjunction with NGS to identify epigenetically modified regions of the genome.
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.