Our Barcodes for Sequencing service provides a seamless, prepaid solution for Sanger, plasmid, and amplicon sequencing needs. Leveraging our extensive experience and advanced automation technology, we offer barcodes that streamline the sequencing process, minimizing errors and enhancing efficiency.
This fast, reliable, and convenient service is designed to optimize your experience using our fully automated workflows that ensure precise sample assignment, convenient online order tracking, and secure data retrieval directly from your CLIMS account.
Our Barcodes for Sequencing service provides handy sample labeling and a more convenient payment option for all sequencing products submitted in tubes or plates. Barcode labels can easily be shared with colleagues and managed through the Payment Center within CLIMS, our online ordering/data management system.
We provide two types of barcode options at GENEWIZ:
1. Prepaid Barcodes: Secure flexibility, convenient logistics, automated sample processing workflows, and easy online order tracking throughout the entire process. Enjoy minimized paperwork and simplified invoicing, boosting your sequencing efficiency.
2. Non-Prepaid Barcodes: Use these with any of our sequencing services for a flexible selection of your specific needs. Activate Non-Prepaid Barcodes in your CLIMS account to choose and utilize the desired sequencing service.
Our barcoding service is designed to optimize your experience by streamlining sample management and enhancing workflow efficiency. Simply order the barcodes from your CLIMS account and submit samples at your nearest dropbox or via a local courier.
Avoid delays in your Sanger data delivery by reviewing our instructions for barcode labeling.
Barcode-driven automated workflows enable flawless and convenient sample processing. For Vario plate, digital barcodes will be deposited into your account - your Vario Plate will be labeled with a physical label at our Azenta lab.
Pre-Defined Vario Plate Sequencing Service is for sequencing plasmids or PCR fragments in 96-well plates and is our most flexible service in terms of the number of templates and the number of primers per template that can be submitted using a 96-well plate.
For each template submitted in a well of a Vario Plate, you can send / select up to four primers that are used for the sequencing of your template.
The invoicing occurs reaction-based instead of plate-based.
Speed: Fully-automated workflow processes, quick turnaround times
Convenient: Easy online ordering with no need for submitting complicated order forms
Accurate: Reliable way to accurately read encoded information, eliminating the possibility of human error
Secure: Safe and secure data download from your CLIMS account
Today’s molecular biologists have many options to interrogate their genome or transcriptome of interest. Next generation sequencing (NGS), quantitative PCR (qPCR), digital PCR (dPCR), and Sanger sequencing are used widely in genomics; however, choosing the best tool for your project isn’t always intuitive. To learn more about the method best suited for your project goals, use our interactive selection guide as well as the practical information about PCR + Sanger, qPCR, dPCR, and NGS approaches below.
Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.
Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data.
Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template