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Next Generation Sequencing
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Sequencing OnlyRNA-SeqProteomics NEWHigh Throughput Gene Expression Screening NEWSingle-Cell SequencingExome SequencingAmplicon Sequencing ServicesWhole Genome SequencingCRISPR ValidationTargeted SequencingMetagenomics SolutionsEpigenomicsAntibody Discovery NEWImmunogenomicsDigital Spatial ProfilingAAV Genome SequencingWhole Plasmid Sequencing: Plasmid-EZ NEW
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Sanger Sequencing
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Sanger-EZ NEWAAV-ITR Sanger SequencingService & OptionsBarcodes for SequencingLogistic Options
Direct Colony Sequencing NEWPrimer Walking
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PCR + Sanger Services
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Bacterial and Fungal IdentificationMutation AnalysisSNP GenotypingPCR Plus Sequencing CRISPR Analysis PackagecDNA & gDNA Verification ServicesYeast Colony SequencingFragment AnalysisQuantitative PCR & Digital PCR NEW
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DNA & RNA Synthesis
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Gene SynthesisAntibody SynthesisAAV Plasmid SynthesisGene FragmentsSynthetic DNA LibrariesCRISPR Construct SynthesisssDNA SynthesisDNA CloningSite-Directed Mutagenesis
Viral Packaging NEWRecombinant Antibody Production NEWCustom mRNA Synthesis NEW
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Oligo Synthesis Services
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Custom DNA And RNA Oligos
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Plasmid DNA Preparation
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Standard Plasmid DNA PrepTargeted Yield Plasmid DNA PrepAAV Plasmid Preparation
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Preclinical and Clinical Services
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Clinical ServicesMicroarray Solutions NEWCLIA Variant Confirmation (PCR + Sanger)CLIA Sanger SequencingCLIA Whole Exome SequencingCLIA Whole Genome SequencingGLP-Compliant OverviewGLP Confirmatory SequencingGLP SNP Mutation AnalysisGLP Plasmid PrepGLP Nucleic Acid ExtractionBiofluid ProcessingCLIA Integration Site Analysis NEW
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GENEWIZ Sanger Sequencing SERVICES
Sanger sequencing at GENEWIZ is easy and straight-forward. Our Sanger sequencing products are designed for a variety of starting materials submitted in tubes or plates. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, biotechnology, and government institutions. Sequencing results can be delivered online within hours, depending from service ordered.
Place an Order
Service & Options for Sanger Sequencing
Azenta offers two service types for Sanger sequencing of PCR products or plasmids: Pre-Mixed or Pre-Defined, both available for tubes and plates. Our barcode-driven automated workflows enable flawless and convenient sample processing. To determine the service that is best for your samples, please see the table and the descriptions for each service below. To ensure the best results, please submit your samples according to our Sample Submission Guidelines for each service type.
If you need help choosing your optimal Sanger service option, please contact us:
Email: sanger.europe@azenta.com | Phone: +49 (0) 341 520 122-41
Email: genomics.service.uk@azenta.com | Phone: +44 (0) 1279 873837
Pre-Mixed Tube
For sequencing plasmids or purified PCR products, this service is the most economical choice. You provide your template and primer mixed in the same tube.
Pre-Defined Tube
You submit your template in a tube and we will mix template and a defined primer when your samples are received. You can choose from different available FREE Universal Primers or provide your own, labeled with a Purple, primer barcodes
Custom
This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, a GENEWIZ scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.
Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.
Submit template and primer separately according to our Sample Submission Guidelines.
Note: A GENEWIZ Universal Primer can be used in this service at no charge.
Difficult Template Sequencing
Revolutionize your sequencing experience with our Difficult Template sequencing service, specifically designed for challenging templates featuring GC-rich regions or intricate secondary structures like hairpins. With a success rate surpassing standard workflows by over double, researchers can now save at least a day in their process. Versatile and efficient, this protocol accommodates various DNA types without the need for additional templates, offering unparalleled convenience and reliability.
Learn more about our proprietary Sanger sequencing service – Difficult Template Sequencing
Features & BENEFITS
Speed
Fully-automated workflow processes, quick turnaround times
Convenient Logistics
Easy online ordering with no need for submitting complicated order forms
Best-in-Class Difficult Template Protocols
For hairpins and GC-rich DNAFree Vector Primers
Wide selection of GENEWIZ Universal Primers are available for most standard vectorsAccurate
Reliable way to accurately read encoded information, eliminating the possibility of human error
Secure
Safe and secure data download from your CLIMS account
Technology
Analysis using ABI 3730xl DNA Analyzers for capillary electrophoresis and fluorescent dye terminator detectionHOW TO ORDER
Email EU: sanger.europe@azenta.com | Phone: +49 (0)341 520 122-41
Email UK: genomics.service.uk@azenta.com | Phone: +44 (0) 1279 873837
GENEWIZ from Azenta Life Sciences
European GENEWIZ Headquarters
GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41