Confirmatory Sequencing & cell bank characterization

 

Trust Azenta Life Sciences, formerly GENEWIZ to manage your project from assay design to data analysis. Simply submit your samples and let us do the rest!

Our experienced scientists will design, develop, and optimize your assays to verify the identity of your sequences and provide you with the final data. We can even utilize samples submitted from existing Azenta Next Generation Sequencing projects.

Azenta’s Sanger-based confirmatory sequencing services can be customized to detect your endogenous or recombinant transcripts/genes, support cell line development, monoclonal antibody heavy chain/light chain sequence confirmation, and transfection verification.

  

Get Rewarded for Your Referrals

Unlock 20% off your next genomics order*

*Terms & conditions apply

Confirmatory Sequencing Workflow

1. Submit Samples

 

2. Primer Design + Assay Development

3. Extraction

4. PCR Amplification

5. Sanger Sequencing

6. Data Analysis

Deliverables

Submit your samples and GENEWIZ will take care of the rest!

Our experienced scientists design, develop, and optimize assays to verify the identity of your sequences and provide you with:

 

  • Chromatogram file (.ab1)
  • Analysis report, including identification of mutations or discrepancies
  • Sequence file (.seq)
  • Consensus sequence of results (.seq)
  • Analysis report, including identification of mutations or discrepancies


    • Applications of Confirmatory Sequencing

    Sanger-based confirmatory sequencing of both DNA and RNA/cDNA templates has been utilized to support the following applications:

    CRISPR Confirmation


    Confirmation of target CRISPR integrations (& identification of off-target effects)

    Next Generation Sequencing Confirmation


    Confirmation of mutations identified by NGS screens

    Antibody Sequencing Confirmation


    Confirmation of heavy-chain/light-chain sequences for monoclonal antibodies

    Cell Line/Cell Bank Confirmation


    Genotyping & confirmation of engineered cell lines (cell line ID/stability)

    Gene Synthesis Product Confirmation


    Confirmation of clones and gene editing products    

    Transfection Verification


    Confirmation of genomic integrations in cell lines & viral vectors (lentivirus or AAV)



    Find out more about our plant genetics services OR inquire about unique sample type:

     

      



    Features & Benefits

    Complete Solution: Nucleic Acid extraction to Sanger sequencing & data analysis

     

    Complete Sequence Coverage: Our team ensures complete sequencing of your region of interest, including high GC content regions, and TA Cloning, as needed

    Superior Support: Dedicated GENEWIZ scientist, expert technical support, and consistent, transparent communication with weekly project updates

    Sample flexibility: Our team has experience working with many sample types including: cell pellets, tissue samples, gDNA, RNA/cDNA, plasmids, FFPE samples and more.



    Ensure Complete Plasmid Validation on Your First Attempt

    with Oxford Nanopore Sequencing

     

     

    Technical Resources

    Related Services

    How to Order

    *Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

    Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)


    GENEWIZ from Azenta Life Sciences

    European GENEWIZ Headquarters

    GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41

    Copyright © 2024 All Rights Reserved.