Next Generation Sequencing: Amplicon-EZ

 

Amplicon sequencing made easy!

  • Easy on your budget – as low as €53/£47 per sample
  • Easy to order – checkout online in minutes
  • Easy to submit samples – use a Azenta Life Sciences drop box
  • Easy to analyze data – interactive reports with intuitive design
  • Easy on your deadline – results in 8 business days*

Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast. Applications include CRISPR screening, variant genotyping, antibody discovery, and much more.

To make our Amplicon-EZ service even easier and quicker for European customers, processing is now available at our European Genomics Headquarters in Leipzig, Germany.


   
 

Amplicon Sequencing in 8 Days*

1. Sample submission

  • No minimum sample number
  • Convenient sample submission via dropboxes

2. Library Preparation

  • 150-500 bp products accepted
  • Submit with or without lllumina® partial adapters

3. Sequencing

  • -50,000 reads per sample
  • Full sequence coverage with 2x2S0 bp sequencing

4. Data Analysis

  • Interactive variant report
  • Unique sequence identification and abundance analysis

*Samples are processed at our European Genomics Headquarters in Leipzig, Germany in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).

APPLICATIONS: CRISPR SCREENING & MORE


CRISPR screening and validation - on/off-target mutations

Somatic variant analysis

Taxonomic identification - 165, 185. or ITS regions

Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb
genes

Clone verification and screening

Diversity analysis – synthetic DNA libraries

CRISPR Screening with Amplicon-EZ

Digital Spatial Profiling Stains

1. Edit cells with CRISPR

 
Digital Spatial Profiling Target Areas

2. Amplify target region from edited cell populations

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 
Separate ROIs

4. Analyze results to find wells with desired mutations**

 

CRISPR Validation with Amplicon-EZ

Digital Spatial Profiling Stains

1. Isolate clones from postitive wells (see CRISPR screening)

 
Digital Spatial Profiling Target Areas

2. Amplify target region from clones

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 
Separate ROIs

4. Validate edits and find homozygous mutants**

 

**View our interactive report (login required)


Want Azenta to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.

Illumina
PacBio
10x Genomics
NanoString
Nanopore
Olink


How To Order


Email | Phone +49 (0)341 520 122-41